UVP DigiDoc-It User Manual

Life Science Software Installation and User Instructions Doc-It®LS Image Acquisition Software Doc-It®LS Image Analysis Software VisionWorks®LS

UVP LIFE SCIENCE SERIES SOFTWARE - 10 - COPY OF THE SALES RECEIPT OR PACKING SLIP. LICENSOR’S ENTIRE LIABILITY AND LICENSEE’S EXCLUSIVE REMEDY WILL

ERROR! REFERENCE SOURCE NOT FOUND. - 100 - RULERS You will find rulers at the top and left of each Image window. The rulers show the width and h

ERROR! REFERENCE SOURCE NOT FOUND. - 101 - 5. To change the color, font size and formatting (bold, italic, underline), click once on the text to ch

ERROR! REFERENCE SOURCE NOT FOUND. - 102 - the default for the next Text annotation you create. To enter new text, simply start typing and the exist

ERROR! REFERENCE SOURCE NOT FOUND. - 103 - checked; then click on any part of the annotation. If the annotation is not near any other annotations

ERROR! REFERENCE SOURCE NOT FOUND. - 104 - • Color: You can either pick a color from the list or choose Custom Color at the bottom to pick any exis

ERROR! REFERENCE SOURCE NOT FOUND. - 105 - OVERVIEW LS Software keeps various sorts of information about an image. This Image Information includes:

ERROR! REFERENCE SOURCE NOT FOUND. - 106 - To Enter Notes 1. Display the Image Information window as described above. 2. In the Properties tab, ty

ERROR! REFERENCE SOURCE NOT FOUND. - 107 - To View Image History 1. If the image is not the foremost image, select it from the Windows menu or cl

ERROR! REFERENCE SOURCE NOT FOUND. - 108 - Some combinations result in different colors than one might expect. For instance, a color pixel with high

CHAPTER TWELVE: PRINTING IMAGE REPORTS - 109 - CHAPTER TWELVE: PRINTING IMAGE REPORTS • Reports o Image Reports o Image Effects o Image History o

CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE - 11 - CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE • Introduction • Minimum System Requ

CHAPTER TWELVE: PRINTING IMAGE REPORTS - 110 - 2. Choose Tools > Reports and then choose the report names from the Reports window. 3. To chang

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 111 - CHAPTER THIRTEEN: FINDING LANES AND BANDS • Overview • Navigation • Finding 1D Gel Lanes and Ban

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 112 - 1D Analysis Plugin Buttons y Find Lanes and Bands: Searches for lanes and bands in the image.

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 113 - The Image Window may display any or all of: y The Region of Interest (ROI) y Lanes (marked by co

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 114 - y Automatically curve y Find Bands y Delete y Properties 1D Analysis Settings To access analys

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 115 - Selecting the Region of Interest 1. From the Tools menu, select Region of Interest. Select the r

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 116 - lanes you have defined. 2. Under Search Options, select whether you want lane width to be constant

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 117 - You can also right click on any lane and select set the band sensitivity. MODIFYING BANDS If th

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 118 - 3. Click on OK. The Lane Profile window closes and bands are now in their new positions. When

CHAPTER THIRTEEN: FINDING LANES AND BANDS - 119 - CLEARING ALL LANE AND BAND INFORMATION If you wish to begin a new analysis of your image, save you

CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE - 12 - y Internet Explorer 6.0 or higher [To determine the version of Internet Explorer, open I

CHAPTER FOURTEEN: MOLECULAR WEIGHT CALIBRATION - 120 - CHAPTER FOURTEEN: MOLECULAR WEIGHT CALIBRATION • Overview • Retardation factor Lines • Mol

CHAPTER FOURTEEN: MOLECULAR WEIGHT CALIBRATION - 121 - You also can access these features using the 1D Analysis Plugin. To Adjust an Automatic Rf

CHAPTER FOURTEEN: MOLECULAR WEIGHT CALIBRATION - 122 - white, and you have created a new Rf Line. 4. To place more Rf lines, follow the same proces

CHAPTER FOURTEEN: MOLECULAR WEIGHT CALIBRATION - 123 - To help you in your analysis, LS provides a library of molecular weight standards. You can a

CHAPTER FOURTEEN: MOLECULAR WEIGHT CALIBRATION - 124 - 3. In the new window, select the standard you wish to copy. Then click on Copy. 4. The Edit

CHAPTER FOURTEEN: MOLECULAR WEIGHT CALIBRATION - 125 - Using the Stretch Factor gives you a weight match on the band up to 0.5% of the weight you a

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 126 - CHAPTER FIFTEEN: QUANTITATION CALCULATIONS • Overview • Background Correction • Concentration Cal

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 127 - 2. On the graph, in the second box below Background Correction, click the down arrow to show the

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 128 - Rolling Disc Picture turning the lane profile graph upside and then rolling a ball over the new

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 129 - To Show the Concentration Graph 1. From the Analysis > 1D Analysis > Master Tools > Conce

CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE - 13 - Depending on the camera drivers that are selected, you may also get the following type of

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 130 - If you do not see the unit type you want in the drop-down menu, LS software allows you to add it

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 131 - Selecting Data Points In the Concentration Window, note that there are three tabs, Graph, Da

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 132 - 3. Click Delete. 4. Click OK. You can access the Concentration window from the 1D Analysis Toolb

CHAPTER FIFTEEN: QUANTITATION CALCULATIONS - 133 - LS software automatically and immediately fits the curve model you choose to the data points as yo

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 134 - CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA • Overview • Lane Information • Ban

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 135 - To View and Use Lane Properties Once you have a lane selected, from Analysis > 1D Ana

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 136 - proportions of the band. Band Properties also offers you the following information: y

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 137 - Displaying Multiple Lanes LS software allows you to display one or more lanes at a time

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 138 - By default, the x-axis displays Pixels and the y-axis displays Intensity. However, af

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 139 - The software also allows you to choose the color of the graph's axes. To do so:

CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE - 14 - • Setting up user accounts is mandatory if you need 21 CFR part-11 support from LS softwa

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 140 - To access this Analysis > 1D Analysis > Master Tools, select Data Explorer. Data

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 141 - To Select Filter Data Fields The top left corner of the Data Explorer window offers a d

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 142 - Any data column can be removed from the report by clicking and dragging that data colum

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 143 - 5. Name the file and click Save. FIXED IMAGE AND ANALYSIS REPORTS Fixed Reports LS

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 144 - To Change General Layout Before Printing 1. To change the margins, click on the Page Se

CHAPTER SIXTEEN: VIEWING & PRINTING 1D GEL DATA - 145 - Analysis Bands The software prints reports detailing band analysis. The Analysis Bands

CHAPTER SEVENTEEN: COLONY COUNTING - 146 - CHAPTER SEVENTEEN: COLONY COUNTING • Overview • Automatic Counting • Manual Counting • Results Window

CHAPTER SEVENTEEN: COLONY COUNTING - 147 - Without any more input, the software will open a new image and recognize the colonies (or objects) prese

CHAPTER SEVENTEEN: COLONY COUNTING - 148 - Once the ROI is adjusted, click Next to proceed. Background flattening – Step 2 of 5 This is a very imp

CHAPTER SEVENTEEN: COLONY COUNTING - 149 - boundaries. Change the filter-size to verify how the image looks by pressing the Flatten Background butt

CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE - 15 - To Edit a new user: Edit the New User by highlighting the user name in the User Configur

CHAPTER SEVENTEEN: COLONY COUNTING - 150 - This lets you choose the class based on an existing object (colony) in the image. First, click on a c

CHAPTER SEVENTEEN: COLONY COUNTING - 151 - Close: This filter fills up spaces between adjacent colonies. Some facts about filters: ‘Size’ signifie

CHAPTER SEVENTEEN: COLONY COUNTING - 152 - The result window The result window has four tabs: • Classes: Provides statistics at the level of cla

CHAPTER SEVENTEEN: COLONY COUNTING - 153 - 15 Max. Density Lum Maximum Density Lum found for this class • Colonies: This section lists all colo

CHAPTER SEVENTEEN: COLONY COUNTING - 154 - Lum Mean Average area of all colonies Average Density Lum over all colonies Std. Dev. Standard Deviat

CHAPTER SEVENTEEN: COLONY COUNTING - 155 - Save Results to Excel: Clicking this option saves the results of first three tabs (classes, colonies and

CHAPTER SEVENTEEN: COLONY COUNTING - 156 - TIPS • Use the Zoom/Pan tool to work with smaller areas for more accurate assessment. • Colonies can be

CHAPTER EIGHTEEN: AREA DENSITY - 157 - CHAPTER EIGHTEEN: AREA DENSITY • Purpose • Area Density Options • Steps to Follow • Saving the Results •

CHAPTER EIGHTEEN: AREA DENSITY - 158 - Start Area Density Click this button to start the area density procedure. This will prompt the user to defi

CHAPTER EIGHTEEN: AREA DENSITY - 159 - Area Density Results – Density List (Tab) Region Total Density Sum total of intensities of all pixels with

CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE - 16 - Column Heading Description User Name Unique Identification name for a particular user. T

CHAPTER EIGHTEEN: AREA DENSITY - 160 - Area Density Results Statistics (Tab) Min Minimum value of attribute listed in corresponding columns, among

CHAPTER EIGHTEEN: AREA DENSITY - 161 - f. Hide user background Start Area Density and Define the Region 1. From the Area Density plugin, click St

CHAPTER EIGHTEEN: AREA DENSITY - 162 - Define Background By default, each area has a separate background. It is equal to the sum total of the perime

CHAPTER EIGHTEEN: AREA DENSITY - 163 - 4. (Optional) Create an Amount Calibration Curve by clicking on the corresponding button on the Area Densit

CHAPTER EIGHTEEN: AREA DENSITY - 164 - You can also Update the results from the Table menu on the Area Density Results window. SAVING AND PRINTING T

CHAPTER EIGHTEEN: AREA DENSITY - 165 - of lighting and imaging. These errors may change the mapping of original input intensity value to higher or l

CHAPTER EIGHTEEN: AREA DENSITY - 166 - To Calibrate the Intensity To add points on the Intensity Calibration Curve, click Add on the Points List Co

CHAPTER EIGHTEEN: AREA DENSITY - 167 - Changes are reflected on the curve after the window is closed. Special Cases of Free Form Curves Calibrati

CHAPTER EIGHTEEN: AREA DENSITY - 168 - Monotonic Calibration A curve of the following type (left) can have two values of input (X) for some val

CHAPTER EIGHTEEN: AREA DENSITY - 169 - To Save Calibration: From the Area Density Results window, click on File > Save Calibration and save the

CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE - 17 - MATRIX OF USER RIGHTS Depending on the privileges for the user that has logged onto w

Chapter Nineteen: Towards 21 CFR Part-11 Compliance - 170 - Chapter Nineteen: Towards 21 CFR Part-11 Compliance • Purpose • Features • Usage Purp

Chapter Nineteen: Towards 21 CFR Part-11 Compliance - 171 - 3. Events are listed in the left column. Click on each event to view the entry detail

Chapter Nineteen: Towards 21 CFR Part-11 Compliance - 172 - SECURE USER ACCOUNTS Refer to Chapter 1 discussing Secure User Accounts for detailed

- 173 - other liability, whether in an action of contract, tort or otherwise, arising from, out of or in connection with GraphicsMagick or the use

- 174 - SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. 4. Magick++ Copyright The C++ API known as "Magick++", and whic

- 175 - This copy of the libpng notices is provided for your convenience. In case of any discrepancy between this copy and the notices in the file

- 176 - Dave Martindale Guy Eric Schalnat Paul Schmidt Tim Wegner The PNG Reference Library is supplied "AS IS". The Contrib

- 177 - INCIDENTAL, INDIRECT OR CONSEQUENTIAL DAMAGES OF ANY KIND, OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS OF USE, DATA OR PROFITS, WHETHER

- 178 - APPENDIX 2: GLOSSARY Artifact: In imaging, a flaw caused either by the imaging process or by the hardware itself. For example, dust on t

- 179 - Microscopy: The use of or investigation with a microscope. Monochrome: Black-and-white, with shades of gray. Doc-It cameras capture 256

CHAPTER TWO: SOFTWARE REGISTRATION AND ACTIVATION - 18 - CHAPTER TWO: SOFTWARE REGISTRATION AND ACTIVATION • Overview • Single-user license regist

- 180 - INDEX 1 1D Analysis 47 1D Analysis Menu 111 1D Gel 111 Bands 117 Concentration Calculation 126 Lane Profile Graph 136 Menus 111 Repor

- 181 - Change General Layout Before Printing 142, 143, 145 Chemi Camera 50 Chiseled in Stone 93 Clear All Lanes 111, 119 Cohu Camera 48 Colo

- 182 - Histogram 45 History 114 How Computer Images Work 107 I Image 33 Caption 33 Depth 178 Editing 81 History 106 Information 105 Loading 77 S

- 183 - P Passwords Create,Reset 13 Paste 84 into Other Software Packages 83 Paste Special 84 Undoing 81 Periodic Noise Removal 92 Pixel 179 Und

- 184 - Stretch Factor 124 T Tabular Layout window 139, 142 Tabular Layouts Window 140 Tabular Reports 142 Targa 78, 179 Templates 69 Text Ann

CHAPTER TWO: SOFTWARE REGISTRATION AND ACTIVATION - 19 - 4. After selecting the Setup.exe, you will be presented with a simple Installation Wizar

UVP LIFE SCIENCE SERIES SOFTWARE - 2 - TABLE OF CONTENTS CHAPTER ONE: WELCOME TO LIFE SCIENCE (LS) SOFTWARE ...11 Intr

CHAPTER TWO: SOFTWARE REGISTRATION AND ACTIVATION - 20 - 7. The next screen will verify the “Port” number to be used. It will be necessary to not

CHAPTER TWO: SOFTWARE REGISTRATION AND ACTIVATION - 21 - 9. Next it will be necessary to obtain the “Server” computer’s IP Address. This address

CHAPTER TWO: SOFTWARE REGISTRATION AND ACTIVATION - 22 - 12. You will now see the IP Address listed. Please note this down, as it will be used on t

CHAPTER TWO: SOFTWARE REGISTRATION AND ACTIVATION - 23 - 15. Next you will see a message letting you know the License Server installation/configura

CHAPTER FOUR: PLUG-IN MODULES - 24 - CHAPTER THREE: WORKSPACE NAVIGATION • Main Window • Menus • Toolbars • Plug-in Modules • Image Windows • Stat

CHAPTER FOUR: PLUG-IN MODULES - 25 - Customizing the toolbars is explained later in the toolbars section of this chapter. To Show or Hide the

CHAPTER FOUR: PLUG-IN MODULES - 26 - When a camera plugin is hidden, you cannot control the camera or change the exposure settings. MENUS LS Softwa

CHAPTER FOUR: PLUG-IN MODULES - 27 - • View Menu: Contains commands that show and hide various main-window features and commands that affect how

CHAPTER FOUR: PLUG-IN MODULES - 28 - y Image Menu: Contains commands that show the Scan options, extract frames, merge images, multiple color chann

CHAPTER FOUR: PLUG-IN MODULES - 29 - y Window Menu: Contains commands to organize Image windows. Shows the arrangement of the image window and s

UVP LIFE SCIENCE SERIES SOFTWARE - 3 - Ftp Transfer ...

CHAPTER FOUR: PLUG-IN MODULES - 30 - • Reduce to Mono: Converts the image to a single-channel (monochrome) image from a multi-channel (color) image

CHAPTER FOUR: PLUG-IN MODULES - 31 - Rearranging Toolbars You can rearrange the order of the individual toolbar sections by placing the mouse arrow

CHAPTER FOUR: PLUG-IN MODULES - 32 - Rearranging Plug-in Modules You can position the modules anywhere on your screen and several ways you can selec

CHAPTER FOUR: PLUG-IN MODULES - 33 - Position the plug-in module at the left of the workspace Position the plug-in module at the right of the work

CHAPTER FOUR: PLUG-IN MODULES - 34 - Organizing Image Windows y Images can be visible in work area with either tabbed layout, shown above or casca

CHAPTER FOUR: PLUG-IN MODULES - 35 - click the right mouse button and click View Best Fit. Context Menu Commands A context (shortcut) menu appears

CHAPTER FOUR: PLUG-IN MODULES - 36 - CHAPTER FOUR: PLUG-IN MODULES • Log Viewer • Digital Video Player • Olympus Camera • Hamamatsu Camera • Hist

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 37 - DIGITAL VIDEO PLAYER (DVP) The Digital Video Player (DVP) clubs multiple images in a single file

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 38 - Controls on the 3D plot window Viewpoint tab Controls on this tab let the user set the correct an

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 39 - IMAGE FILTERS This plugin offers several types of image filters to make changes to an image. Fi

UVP LIFE SCIENCE SERIES SOFTWARE - 4 - Convert ...

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 40 - ZOOM/PAN By Zooming you can magnify part of the image making small details more visible. Once you

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 41 - 4. Click the Magnifying Glass button. 5. The mouse becomes a magnifying glass. Click anywhere

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 42 - IMAGE CONTROLS This plugin offers features to control how an image looks. None of the Effects m

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 43 - Contrast Enhanced Inverted To Change Brightness, Contrast, Gamma Or Invert 1. If the Image Con

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 44 - LS package supplies built-in pseudocolor spectrums: y Over-Saturation: Colors pixels in top 5%

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 45 - To Remove a Pseudocolor 1. If the Pseudocolor plugin is not showing, choose Plugins > Pseudoc

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 46 - AREA DENSITY The Area Density plugin can be used to carry out precise quantitative calculations

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 47 - 1D ANALYSIS This plugin offers easy access to the analysis functions instead of using the menus

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 48 - HAMAMATSU CAMERA If using the Hamamatsu camera, open the Hamamatsu Camera plug-in module. This p

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 49 - COLONY COUNTING This plugin offers easy access to the analysis instead of using the menus. The m

UVP LIFE SCIENCE SERIES SOFTWARE - 5 - Overview...

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 50 - BIOCHEMI HR/CHEMI HR 410 CAMERAS If using the BioChemi HR or Chemi HR 410 Cameras), open the Che

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 51 - DARKROOM AND LENS If using the motorized lens, open the Darkroom and Lens plug-in module. This p

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 52 - CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES • Overview • Darkroom connectivity • Selecting a

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 53 - You will see the status of connectivity of the darkroom.If it says “BioSpectrum Hardware Detecte

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 54 - Darkroom Template To Create a New Template 1. Click New from the BioSpectrum Template window.

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 55 - OPTICHEMI This section pertains to several UVP cameras: OptiChemi Camera To view the plug-in f

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 56 - Hamamatsu Camera Plug-In Integration display option • On-Chip Integration: When this radio-butt

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 57 - picture. Capture Information: Shows camera status and capture progress. Preferences: Shows the Ha

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 58 - Binning Modes Binning is an advanced feature provided by these UVP cameras. “Binning” literally m

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 59 - These five pictures will be placed inside a sequence file, and you can use the Digital Video Play

UVP LIFE SCIENCE SERIES SOFTWARE - 6 - APPENDIX 2: GLOSSARY...178

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 60 - Current Black Level: Stacking frames also stacks noise along with it. To clip noise from each ima

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 61 - BioChemi HR/Chemi HR 410 Camera Plug-In Integration display option • On-Chip Integration: When

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 62 - Preview: Click on this button to see a preview of your sample, in order to ensure that the camera

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 63 - Cohu Camera Plugin Cohu Capture Template Templates are a great time-saver, regardless of the type

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 64 - To ‘Save’ a Template, click on ‘Template’ button. That brings up a window asking you to give a na

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 65 - which brings up a window that looks like the following: Constant Time Intervals: Use this when y

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 66 - long exposure. In this mode, LS software does “stacking” of frames i.e. it adds the corresponding

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 67 - Canon Capture Plug-in The UVP Canon digital camera capture plugin provides simple camera contro

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 68 - 1. Place a UVP Focus Target on the transilluminator. If there are no bright or contrasting areas

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 69 - Zoom Adjustment with the Calibration and Lock Focus While imaging with the focus locked, the Op

UVP LIFE SCIENCE SERIES SOFTWARE - 7 - END USER LICENSE AGREEMENT PLEASE READ THE FOLLOWING AGREEMENT CAREFULLY: This Agreement is between UVP, LLC

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 70 - To Create a New Template 6. On the Canon Camera Plugin click the drop-down Templates menu. The

CHAPTER FIVE: ACQUIRING AND MANAGING IMAGES - 71 - scanners support TWAIN.) TWAIN helps scanners provide their own software interface during scannin

CHAPTER SIX: DIGITAL VIDEO PLAYER - 72 - CHAPTER SIX: DIGITAL VIDEO PLAYER • Purpose • How to access the player • Player Features • Player Options

CHAPTER SIX: DIGITAL VIDEO PLAYER - 73 - BUTTON FUNCTIONALITY This button allows viewing the next image. This button allows viewing the previous

CHAPTER SIX: DIGITAL VIDEO PLAYER - 74 - Frame Rate This is the interval (in milliseconds) between two consecutive images that are displayed. For

CHAPTER SIX: DIGITAL VIDEO PLAYER - 75 - The other two ways this action can be initiated is: If the player is open, click on Extract/Delete from t

CHAPTER SIX: DIGITAL VIDEO PLAYER - 76 - Controls: Add button: Click on ‘Add’ button to find files from the hard-disk to merge. Existing files in

CHAPTER SEVEN: LOADING, SAVING AND TRANSFERRING IMAGES - 77 - CHAPTER SEVEN: LOADING, SAVING AND TRANSFERRING IMAGES • Loading Images • Saving Im

CHAPTER SEVEN: LOADING, SAVING AND TRANSFERRING IMAGES - 78 - 1. From the File menu choose Save. The Save window will appear. You can also use

CHAPTER SEVEN: LOADING, SAVING AND TRANSFERRING IMAGES - 79 - PNG: Portable Network Graphics, a common image format. PNG is a lossy compression for

UVP LIFE SCIENCE SERIES SOFTWARE - 8 - LICENSEE’S EXCLUSIVE REMEDY WILL BE REPLACEMENT OF THE DEFECTIVE SOFTWARE AND RELATED DOCUMENTATION OR REFUND

CHAPTER SEVEN: LOADING, SAVING AND TRANSFERRING IMAGES - 80 - Configure the FTP Connection Settings 1. From the File > Preferences > Miscel

CHAPTER EIGHT: IMAGE EDITING - 81 - CHAPTER EIGHT: IMAGE EDITING • Overview • Undo and Redo • Using Selection Tools • Copy • Paste • Paste Special

CHAPTER EIGHT: IMAGE EDITING - 82 - by selecting its title from the Windows menu. 2. From the Edit menu, click Redo. The last change will reappear.

CHAPTER EIGHT: IMAGE EDITING - 83 - Magic Wand: Marks the area automatically on the image. Click just once inside the region you are interested and

CHAPTER EIGHT: IMAGE EDITING - 84 - packages that support images. You can also paste the clipboard contents back on an image using either the Paste

CHAPTER EIGHT: IMAGE EDITING - 85 - The following merge modes are available in all LS Software: Blend: mixes the incoming image with the current

CHAPTER EIGHT: IMAGE EDITING - 86 - incoming image, even if that area is off of the edge of the existing image. This allows you to compose "mos

CHAPTER NINE: IMAGE FILTERS - 87 - CHAPTER NINE: IMAGE FILTERS • Overview • Rotate • Flip Horizontally or Vertically • Resize • Reduce to Mono

CHAPTER NINE: IMAGE FILTERS - 88 - y Rotate: Rotates the image around its center, useful for aligning images taken with a crooked gel. y Flip Hori

CHAPTER NINE: IMAGE FILTERS - 89 - Embossed Rotated ROTATE Rotate an image by an arbitrary number of degrees. You might use it to correct fo

UVP LIFE SCIENCE SERIES SOFTWARE - 9 - END USER LICENSE AGREEMENT PLEASE READ THE FOLLOWING AGREEMENT CAREFULLY: This Agreement is between UVP, LLC

CHAPTER NINE: IMAGE FILTERS - 90 - To Rotate an Image by an Exact Number of Degrees You can also rotate the image by an exact number of degrees. For

CHAPTER NINE: IMAGE FILTERS - 91 - To Resize an Image 1. Choose Image > Filters > Resize. The Resize window will appear. 2. Select the des

CHAPTER NINE: IMAGE FILTERS - 92 - To Convert an Image Bit and/or Color Depth 1. If the image is not the foremost image, select it from the Window

CHAPTER NINE: IMAGE FILTERS - 93 - and it will remove them. Second, the mathematics of the operation can cause some edge pixels to be identified as

CHAPTER NINE: IMAGE FILTERS - 94 - Emboss can be performed from any of eight cardinal directions. Conventionally, these are referred to as North (u

CHAPTER NINE: IMAGE FILTERS - 95 - DUPLICATING IMAGES This command creates an identical copy of the foremost image. The new copy will appear in its

ERROR! REFERENCE SOURCE NOT FOUND. - 96 - CHAPTER TEN: ANNOTATIONS • Overview • Viewing and Hiding Annotations • Types of Annotation • Spatial Ca

ERROR! REFERENCE SOURCE NOT FOUND. - 97 - y Image Rulers: Default to pixel units and can be spatially calibrated for image measurements. y Creatin

ERROR! REFERENCE SOURCE NOT FOUND. - 98 - y Rectangle: Rectangle annotations allow you to draw a rectangular frame around part of the image. You ca

ERROR! REFERENCE SOURCE NOT FOUND. - 99 - 2. Find the feature in the image for which you know the metric size. Click one edge of the feature. 3. M